Representative example of using HLA restriction data derived from SALs to aid in VST selection. (A) Schematic representation of the SAL assay. (B) Bar graphs representing the CD8⁺IFN-γ⁺ and CD4⁺IFN-γ⁺ cell populations by intracellular flow cytometry after the completion of the SAL assay. These data are for product 2777-437B, which was then given to patient 414. The VST only column indicates VST products cocultured with Pepmix rather than cocultured with Pepmix-loaded SALs. (C) ELISpot data from patient 414 showing increase in EBV-directed T cells in the peripheral blood with a concurrent decrease in viral load.