Impact of miR-223-3p modulation on thrombopoiesis. Relative number of platelets in the cell culture (A) and proplatelet swellings per MK (B), after miR-223-3p upregulation (left) or downregulation (right), compared with the negative control. (C) Representative bright-field microscopy images (ImageXpress micro confocal high-content imaging system microscope, ×20 original magnification; scale bar, 20 μm) of human MKs forming proplatelet network derived from CD34⁺ cells in negative control (left) and miR-223-3p downregulation (right) conditions. White arrows show proplatelet swellings. Data are from 3 to 7 independent experiments. ∗P < .05. ns, non-significant.