TMEM16F is a direct target of miR-223-3p. (A) TMEM16F mRNA expression levels in platelets after miR-223-3p upregulation (left) or downregulation (right). (B) Relative luciferase activity after the co-transfection of pmirGLO-3’UTR_TMEM16F WT or MUT with a miR-223-3p mimic and/or an inhibitor in HT1080 cells. Data are from 4 to 6 independent experiments. ∗P < .05; ∗∗P < .01. MUT, mutant; ns, non-significant; WT, wild type.