Platelet-specific PKM2-deficient mice were less susceptible to the development of venous thrombosis. (A) Thrombus incidence in PKM2^WT (PKM2^fl/fl) and PKM2^Plt-KO (PKM2^fl/flPF4Cre⁺) mice 48 hours after IVC stenosis. (B) Representative IVC thrombi harvested from PKM2^WT and PKM2^Plt-KO mice 48 hours after IVC stenosis (top); and quantified thrombus weight and thrombus length (n = 10-11 per group; bottom). Values are mean ± standard error of the mean (SEM); Mann-Whitney U test. (C) CitH3 levels were measured in the thrombus isolated from PKM2^WT and PKM2^Plt-KO mice. Representative western blot for CitH3 is shown (upper), and quantitative data are given (lower). β-actin was used as a loading control (n = 4 per group). Values are mean ± SEM; unpaired Student t test. (D) Fold increase in contraction (left) and percent of relaxation (right) measured by wire myography in IVCs isolated from PKM2^WTand PKM2^Plt-KO mice 48 hours after IVC stenosis. Phenylephrine (0.5 mM) and acetylcholine (1.62 mM) were used for contraction and relaxation, respectively (n = 8-9 per group). Values are mean ± SEM; Mann-Whitney U test.