Figure 3.
Pharmacological inhibition of PKM2 dimerization reduces susceptibility to acute venous thrombosis and improves vein wall response in the WT mice. (A) Thrombus incidence in vehicle- or ML265-treated mice 48 hours after IVC stenosis. (B) Representative IVC thrombus (top) harvested 48 hours after stenosis from vehicle- or ML265-treated mice (50 mg/kg) is shown, along with quantified thrombus weight and thrombus length (n = 12 per group; bottom). Values are mean ± SEM; Mann-Whitney U test. (C) The level of CitH3 was measured in the thrombus isolated from vehicle- and ML265-treated mice. A representative western blot for CitH3 is shown. β-actin was used as a loading control (n = 3 per group). Values are mean ± SEM; unpaired Student t test. (D) Fold increase in contraction (left) and percent of relaxation (right) measured by wire myograph (DMT 610M) in IVCs isolated from vehicle- and ML265 (50 mg/kg)-treated mice 48 hours after IVC stenosis. Phenylephrine (0.5 mM) and acetylcholine (1.62 mM) were used for contraction and relaxation, respectively (n = 8-9 per group). Values are mean ± SEM; unpaired Student t test and Mann-Whitney U test are used for the fold increase in contraction and percent of relaxation, respectively.

Pharmacological inhibition of PKM2 dimerization reduces susceptibility to acute venous thrombosis and improves vein wall response in the WT mice. (A) Thrombus incidence in vehicle- or ML265-treated mice 48 hours after IVC stenosis. (B) Representative IVC thrombus (top) harvested 48 hours after stenosis from vehicle- or ML265-treated mice (50 mg/kg) is shown, along with quantified thrombus weight and thrombus length (n = 12 per group; bottom). Values are mean ± SEM; Mann-Whitney U test. (C) The level of CitH3 was measured in the thrombus isolated from vehicle- and ML265-treated mice. A representative western blot for CitH3 is shown. β-actin was used as a loading control (n = 3 per group). Values are mean ± SEM; unpaired Student t test. (D) Fold increase in contraction (left) and percent of relaxation (right) measured by wire myograph (DMT 610M) in IVCs isolated from vehicle- and ML265 (50 mg/kg)-treated mice 48 hours after IVC stenosis. Phenylephrine (0.5 mM) and acetylcholine (1.62 mM) were used for contraction and relaxation, respectively (n = 8-9 per group). Values are mean ± SEM; unpaired Student t test and Mann-Whitney U test are used for the fold increase in contraction and percent of relaxation, respectively.

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