Figure 5. Limiting PKM2 dimerization... | American Society of Hematology

Figure 5.

$Limiting PKM2 dimerization reduces ex vivo thrombus formation in human whole blood at a venous shear rate. (A) Human whole blood pretreated with vehicle or ML265 (150 μM) was perfused over a TF-coated surface for 8 minutes at a shear rate of 500 s–1 in a BioFlux microfluidic flow chamber system; the representative image at 8 minutes (left) and the quantification of thrombus growth (right) on the TF-coated surface over time are shown. Values are mean ± SEM (n = 3 per group); 2-way ANOVA with Tukey multiple comparisons test. (B) Human whole blood pretreated with vehicle or ML265 (150 μM) was perfused over a TF-coated surface for 25 minutes in a custom-made DVT device. Accumulation of blood cells after 25 minutes of blood flow. Blood cells were stained with DiOC6 in green. The left panel shows the representative image, and the right panel shows the quantification of thrombus growth (fraction of total area) at 25 minutes (the end point of the assay). Values are mean ± SEM (n = 7 per group); paired t test.$

Limiting PKM2 dimerization reduces ex vivo thrombus formation in human whole blood at a venous shear rate. (A) Human whole blood pretreated with vehicle or ML265 (150 μM) was perfused over a TF-coated surface for 8 minutes at a shear rate of 500 s^–1 in a BioFlux microfluidic flow chamber system; the representative image at 8 minutes (left) and the quantification of thrombus growth (right) on the TF-coated surface over time are shown. Values are mean ± SEM (n = 3 per group); 2-way ANOVA with Tukey multiple comparisons test. (B) Human whole blood pretreated with vehicle or ML265 (150 μM) was perfused over a TF-coated surface for 25 minutes in a custom-made DVT device. Accumulation of blood cells after 25 minutes of blood flow. Blood cells were stained with DiOC6 in green. The left panel shows the representative image, and the right panel shows the quantification of thrombus growth (fraction of total area) at 25 minutes (the end point of the assay). Values are mean ± SEM (n = 7 per group); paired t test.

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