TLR ligand-induced migration of DCs to CCL19 is impaired by PPAR-γ activation. Peripheral blood adhering monocytes were cultured with GM-CSF and IL-4 in the presence or absence of TGZ. Different TLR ligands were added to the cells 24 hours before analysis (TLR2L Pam₃Cys, TLR3L Poly(I:C), TLR4L LPS, TLR7L R848). The effect of TGZ on DC migration against CCL19 was analyzed using transwell chambers. 2 × 10⁵ DCs were seeded in the upper chamber in triplicates, and the number of migrated DCs was analyzed after 16 hours. ▪ indicates DMSO; ▦, TGZ.