TGZ treatment enhances dextran uptake by DCs stimulated with TLR ligands. Peripheral blood adhering monocytes were cultured with GM-CSF and IL-4 in the presence or absence of TGZ. Different TLR ligands were added to the cells 24 hours before analysis (TLR2L Pam₃Cys, TLR3L Poly(I:C), TLR4L LPS, TLR7L R848). One × 10⁵ DCs were incubated with FITC-dextran for 1 hour at 37°C, washed 4 times, and analyzed immediately on a FACSCalibur. As control, cells were precooled to 4°C and incubated with FITC-dextran for 1 hour at 4°C. Open histograms represent FITC-dextran-treated cells incubated at 37°C; shaded histograms, the controls incubated at 4°C.