Impaired inflammatory cell recruitment in mindin-deficient mice. (A) Leukocyte number in BAL fluids of GBS-infected mice. Wild-type and mindin^-/- mice (n = 8/group) were infected with GBS intratracheally and BAL fluids were collected 24 hours later for differential cell count. (B) Leukocyte number in BAL fluids of influenza virus–infected mice. BAL fluids were collected at 48 hours after intratracheal influenza virus infection for differential cell count (n = 10/group). (C) Leukocyte number in peritoneal cells of thioglycollate-treated mice. Wild-type and mindin^-/- mice (n = 9/group) were intraperitoneally injected with 1 mL of 3% thioglycollate broth. Peritoneal cells were collected at days 1 and 3 for neutrophil and macrophage count, respectively. (A-C) Shown are mean ± SD. ^*P < .05; ^**P < .001. (D) Number of resident macrophages in the peritoneal cavity of wild-type and mindin^-/- mice. Resident macrophages were harvested from untreated mice and counted. Numbers are the averages of peritoneal macrophages from each group.