Neutrophil adhesion to mindin matrix. (A) Static adhesion of BM neutrophils to coated mindin. BM neutrophils (2 × 105/well) were incubated with coated proteins as indicated. The number of adherent cells was counted in at least 3 different fields after washing. FN indicates fibronectin. (B) Static adhesion of activated BM neutrophils to coated mindin. Purified BM neutrophils (3 × 105/well) were stimulated with PMA (100 ng/mL) for 15 minutes and incubated with coated proteins as in panel A. (C) Static adhesion of peritoneal neutrophils to coated mindin. Peritoneal neutrophils (5 × 104/well) from thioglycollate-induced cells were incubated and counted as in panel A. (D) Mindin-mediated neutrophil migration. BM neutrophils were induced to migrate by fMLP with or without coated protein matrix on transwell membranes. Shown are the numbers of neutrophils migrated to the lower chambers. All data are mean ± SD from triplicate samples and representative of 3 experiments. *P < .001; **P < .05.
