Mindin interacts with integrins. (A) Inhibition of mindin-mediated neutrophil migration by anti-integrin antibodies. BM neutrophils from mindin^-/- mice were incubated with indicated anti-integrin mAbs and tested in mindin-mediated migration as in Figure 4D. Shown are the percent of migration in the presence of antibodies with mindin-mediated neutrophil migration without any blocking antibody as 100%. ^*P < .001. Error bars indicate mean ± SD. (B) Expression of integrins on HEK-293 cells. HEK-293 cells were transiently transfected with plasmids encoding integrins α_M plus β₂, α₄ plus β₁, or vector alone. Forty-eight hours later, integrin expression was examined by FACS. (C) Cell adhesion to mindin matrix. HEK-293 cells expressing integrins as indicated were tested for their binding to coated proteins with or without blocking antibodies. Shown are the numbers of adherent cells in each field (mean ± SD). Data are representative of 2 to 3 experiments.