Figure 3.
Proliferative activity induced by PB DCs obtained from control subjects and patients in allogeneic setting. iDCs and mDCs were generated from patients with CD3+ LDGL (n = 5) and cultured with allogeneic CD3+ GLs or allogeneic CD3- GLs, and normal NK cells or T lymphocytes (A). iDCs and mDCs generated from control subjects (n = 6) were cultured with purified CD3+ or CD3- GLs from patients with LDGL and normal allogeneic NK cells or T lymphocytes (B). DCs obtained from patients with CD3+ LDGL induced proliferation of allogeneic normal T and NK cells and CD3- GLs from patients with LDGL, whereas they were not able to stimulate allogeneic CD3+ GLs. Normal DCs stimulated allogeneic NK cells, T lymphocytes, and CD3- GLs from patients with LDGL but were not able to induce proliferation of allogeneic CD3+ GLs. Data represent mean ± SEM.