Impact of IL-2, IFNγ, TNFα, and TcR cross-linking on expression of CXCR1 on CD8⁺ T cells. CD8⁺ T cells were incubated with increasing concentrations of IL-2, IFNγ, or TNFα (A) or with activating αCD3/αCD28 mAbs (B) and cell surface expression of CXCR1 assessed by FACScan analysis. None of the tested cytokines, nor αCD3/αCD28 mAb activation, induced significant changes in expression of CXCR1.