Figure 2.
Images of α251 and control fibrin clots from laser scanning confocal microscopy. Thrombin at a final concentration of 0.9 IU/mL was added to recombinant control or Aα251 fibrinogen at a 4.4-μM final concentration in 20 mM HEPES, pH 7.4, 0.15 M NaCl, 5 mM CaCl2. Images of 3-dimensional reconstructions of optical sections of control (A, B) and α251 (C, D) fibrin networks obtained by laser scanning confocal microscopy. Original magnifications are as follows: panels A and C: 48 × 48 × 48 μm3; panels B and D: 4 × 24 × 24 μm3.
