Figure 4.
Fibrinolysis of α251 and control fibrin clots as visualized by laser scanning confocal microscopy. Series of micrographs showing the dynamic lysis of control (top panels) and α251 (bottom panels) fibrin clots by rtPA (6 nM) and Glu-plasminogen (2.5 μg/mL); clots were prepared as described in Figure 2. Lysis-front motion was visualized by scanning this region of the clot every 2 minutes. The lysis front progressed as a straight and sharp line in both clots with a higher velocity in α251 fibrin as compared with control fibrin. Each micrograph is a reconstruction from optical sections representing a volume of 146 × 146 × 20 μm3. A,D: 2 minutes; B,E: 4 minutes; C,F: 6 minutes.
