Evi1 and Prdm16 mRNA expression is activated by MSCV integrations. Northern blot analysis of Evi1 and Prdm16 expression in immortalized cell lines using probes specific for each gene. β-actin was used as a loading control. Lines BM-62, BM-72, and BM-83 have confirmed Evi1 integrations, while lines BM-60, BM-67, BM-69, BM-75, BM-80, and BM-81 have confirmed Prdm16 integrations. Lines BM-37 and BM-70 have neither integrations.