Figure 4.
Figure 4. Expression of BM9 and BM10 inhibits PEL cell growth and reduces endogenous expression of LANA1 protein. Short-term growth curves for BCBL-1 (A) and BC-1 (B) cells transfected with BM9 (•), BM10 (○), and 4BL (♦) scFvs and selected with zeocyn show growth inhibition with anti-LANA1 scFv expression contrary to 4BL, an irrelevant HIV-specific intrabody. (C) Immunoblotting for LANA1 in BCBL-1 cells transfected with BM9, BM10, and 4BL shows a diminished protein level when BM9 and BM10 are expressed compared with 4BL and untransfected control cells. (D) Distribution of LANA1 and anti-LANA1 scFvs in BC-1 cells with immunofluorescence microscopy after simultaneous detection with mLANA1 and monoclonal HA. LANA1's speckled pattern becomes dispersed when BM9 and BM10 are expressed. scFv is shown in green (FITC) and LANA1 in red (rhodamine). Magnification × 1000.

Expression of BM9 and BM10 inhibits PEL cell growth and reduces endogenous expression of LANA1 protein. Short-term growth curves for BCBL-1 (A) and BC-1 (B) cells transfected with BM9 (•), BM10 (○), and 4BL (♦) scFvs and selected with zeocyn show growth inhibition with anti-LANA1 scFv expression contrary to 4BL, an irrelevant HIV-specific intrabody. (C) Immunoblotting for LANA1 in BCBL-1 cells transfected with BM9, BM10, and 4BL shows a diminished protein level when BM9 and BM10 are expressed compared with 4BL and untransfected control cells. (D) Distribution of LANA1 and anti-LANA1 scFvs in BC-1 cells with immunofluorescence microscopy after simultaneous detection with mLANA1 and monoclonal HA. LANA1's speckled pattern becomes dispersed when BM9 and BM10 are expressed. scFv is shown in green (FITC) and LANA1 in red (rhodamine). Magnification × 1000.

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