Possible role of ST3Gal-VI in selectin ligand formation. WT and ST3Gal-IV^-/- Th1 cells were either untreated or treated with neuraminidase, and rolling on (A) E-selectin or (B) P-selectin was measured. Neuraminidase blocked rolling of cells of both genotypes on both selectins. (A-B) ^**Significantly different from the corresponding group without neuraminidase treatment. (C) qRT-PCR reveals significant induction of ST3Gal-VI mRNA by IL-12 in WT cells but virtually no effect of IL-12 in T-bet^-/- cells. (D) A similar effect on ST3Gal-VI induction is observed with CD4 cells from Stat4^-/- mice, although the magnitude of the effect of Stat4 deficiency is smaller than that for T-bet (note different scales). (C-D) ^**Significantly different from all other groups.