Effects of BAY-11 and FK-506 in primary biopsy-derived LBCL cells. Primary LBCL cells (patients 6 and 7) were left untreated (C; control) or were treated with BAY-11 (B; 1 μM) or FK-506 (F; 5 μg/mL) for 24 and 48 hours. Nuclear extracts purified from 48-hour treated tumor cells were analyzed for NF-κB, NFAT, and Oct-1 DNA binding by EMSA (A). Cytoplasmic extracts from part (A) were analyzed for CD154 and actin protein expression (B). (C) Control and treated cells were counted using the trypan blue exclusion counting method after 24 and 48 hours of treatment. (D) Control and treated cells were analyzed for apoptotic cells using the TUNEL assay. Green fluorescence represents apoptotic cells. (E) Caspase 3 activity was also measured in control and treated cell lysates. The data obtained in Figure 7 were repeated only once due to shortage of primary LBCL cells.