Two-color whole-mount in situ hybridization. (A) CD41 expression does not colocalize with MPO, as judged by the CD41 cells (purple) that do not turn red in the in situ result. (B) On the contrary, the coexpression of CD41 and GFP is supported by the red color (GFP) outcome of originally purple cells (CD41). (C) In addition, coexpression of c-mpl and GFP in the same cells is supported.