Oxidized β₂-GPI increases allostimulatory ability of DCs. Five-day human DCs were stimulated with ox-β₂-GPI (10 μg/mL) (▪), H₂O₂-β₂-GPI (10 μg/mL) (▴), and LPS (100 ng/mL) (•), or left unstimulated (○). After 48 hours, DCs were extensively washed and cultured with allogeneic T lymphocytes (1 × 10⁵ cells/well) for 3 days at a different stimulator-responder ratio (1:16 to 1:256 DC/T). Proliferation of allogenic T cells was measured by ³H-methyl-thymidine incorporation. Data are presented as mean cpm ± SD of triplicate cultures. Results are representative of 11 independent experiments.