Ox-β₂-GPI-treated DCs stimulated allogeneic naive human T cells to produce IFN-γ and IL-10 but not IL-4. Negatively and positively selected CD4⁺CD45RA⁺ T cells (A). Five-day human DCs were stimulated with or without ox-β₂-GPI (10 μg/mL) and LPS (100 ng/mL) for 18 hours. A total of 5 × 10⁴ DCs were used to stimulate 1 × 10⁶ allogeneic naive negatively selected CD4⁺CD45RA⁺ T cells. Activated T cells were expanded with rhIL-2 (30 U/mL) added on day 5. On day 10, polyclonal T-cell lines were stimulated with PMA and ionomycin for 4 hours in the presence of brefeldin A. Cells were stained with anti-hu-CD3PerCP and processed for intracellular labeling with anti-hu-IFN-γ-FITC and anti-hu-IL-4-PE (B) or anti-hu-IL-10-PE (C). The numbers show the percentage of activated CD3⁺ cells producing the cytokine. Samples were analyzed on a FACScan cytofluorimeter using CELLQuest software (Becton Dickinson, Pharmingen Biosciences). The figure shows a representative experiment from 6 with similar results.