Figure 1.
Recombinant CUB-1 polypeptide inhibits UVLWF cleavage under flow conditions. (A) Recombinant CUB polypeptides (100 nM) were incubated with PRP for 10 minutes and then perfused over histamine-stimulated HUVECs at 2.5 dyn/cm2 of shear stress. ULVWF strings formed when buffer was perfused (0% activity), but were cleaved when PRP was perfused (100% activity). The ULVWF strings were detected in PRP that was pretreated with CUB-1 or CUB-1+2, but not when pretreated with CUB-2. (B) The inhibition of ULVWF cleavage by CUB-1 polypeptide was dose-dependent with maximal inhibition at 100 nM. The data are mean ± SEM (Student t test, *P < .01 compared with untreated samples).
