Figure 4.
Recipients of UBM transplants have more IFN-γ-producing NK cells than recipients of TCD transplants. Donor or day +100, donor-derived recipient PBMCs from UBM (n = 24) or TCD (n = 25) transplants were incubated for 24 hours in IL-12 (10 ng/mL) and IL-18 (100 ng/mL). Harvested cells were permeabilized and stained intracellularly with a directly conjugated mAb to IFN-γ. The means, 25th to 75th percentile ranges, and extreme values of the percentages of CD56+/CD3- cells expressing IFN-γ by flow cytometry are shown in the box plot. Results are presented for donors or recipients.