Figure 1.
Figure 1. Bone marrow cells contribute to lymphangiogenesis. A pellet containing 80 ng FGF-2 was implanted into each cornea micropocket of nonirradiated (A-C), irradiated (D-F), and irradiated with EGFP+-transplanted BM (G-I) C57BL/6 mice. At day 7 of implantation, corneal tissues were stained with a combination of anti-CD31 antibodies (A,D,G) and anti-LYVE-1 antibodies (B,E,H). CD31 and LYVE-1+ signals of the same sections were further analyzed for overlapping signals by CLSM analysis (C,F,I). Images were captured using a Plan Neofluar 10 ×/0.3 NA objective lens. Quantification of length (J) and area (K) of LYVE-1+ vessels from 5 to 7 sections.

Bone marrow cells contribute to lymphangiogenesis. A pellet containing 80 ng FGF-2 was implanted into each cornea micropocket of nonirradiated (A-C), irradiated (D-F), and irradiated with EGFP+-transplanted BM (G-I) C57BL/6 mice. At day 7 of implantation, corneal tissues were stained with a combination of anti-CD31 antibodies (A,D,G) and anti-LYVE-1 antibodies (B,E,H). CD31 and LYVE-1+ signals of the same sections were further analyzed for overlapping signals by CLSM analysis (C,F,I). Images were captured using a Plan Neofluar 10 ×/0.3 NA objective lens. Quantification of length (J) and area (K) of LYVE-1+ vessels from 5 to 7 sections.

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