High-power microscopic analysis of the presence of EGFP⁺ cells in corneal vessels. Immunohistologically stained cryosections of FGF-2-implanted corneal tissues were analyzed by CLSM. EGFP⁺ signals were overlapped with LYVE-1 (B-D), VEGFR-3 (F-H), CD31 (J-L), CD34 (N-P), or VEGFR-2 (R-T). Tissue cell nuclei were counterstained with PI (red in panels A,E,I,M,Q). Arrows point to overlapping positive signals of EGFP and vessels. Images were captured with a 40 ×/1.30 NA Plan Neofluar objective lens.