Figure 6.
Incorporated EGFP+ BM cells express CD31 or CD34. Infusion of FACS-sorted VEGFR-3/CD34+/+ (A-D,M-P), VEGFR-2/CD34+/+ (E-H,Q-T), or VEGFR-3/VEGFR-2-/- (I-L,U-X) cells into irradiated mice. The FGF-2-induced vascular structures were immunohistologically analyzed for colocalization of double-positive signals of EGFP/CD31 (A-L) and EGFP/CD34 (M-X). Arrows in panels D, H, L, P, T, and X point to overlapping signals. Images were captured with a 40 ×/1.30 NA Plan Neofluar objective lens.