MN1-TEL enhances growth of myeloid progenitors and blocks their differentiation. (A) MC-2 and -3 of MN1-TEL/Mx1-Cre BM cells. Methylcellulose-based culture (MC) was performed with MN1-TEL/Mx1-Cre BM cells treated with pI-pC or not (MC-1). Colony-forming cells (CFCs) were harvested from the MC-1 and replated into MC-2. The same replating was repeated for MC-3. MT/Cre indicates MN1-TEL/Mx1-Cre; MT, MN1-TEL; Cre, Mx1-Cre. Mean ± SE is shown (n = 3). Experiments repeated in triplicate using different mice yielded identical results. (B) GFP expression in mouse CFCs. GFP expression was analyzed after 10 days in MC-2. (C) M-G staining of CFCs in MC-3. (D) Surface marker analysis of GFP-positive BM cells. BM cells were harvested from 6- to 8-week-old MN1-TEL/Mx1-Cre mice 2 weeks after the last injection of pI-pC. BM cells obtained from age- and sex-matched Aml1-IRES-GFP KI mice were used as controls. Gates for GFP-positive cells were determined by comparing their fluorescence with that of WT BM cells. Numbers indicate the mean ± SE percentage (n = 6).