American Society of Hematology

Figure 3.

$Figure 3. MN1-TEL enhances growth of thymocytes and blocks their differentiation. (A) Increased fraction of S-phase cells in MN1-TEL–positive thymocytes. BrdU was injected in MN1-TEL/Mx1-Cre mice 2 weeks after the last injection of pI-pC or PBS, and 4 hours later thymocytes were harvested and analyzed by FCM analysis after staining with an anti-BrdU antibody. Mean ± SE is shown (n = 10). *P < .05. (B) Surface marker analysis of GFP-positive thymocytes. Thymocytes were harvested from 6- to 8-week-old MN1-TEL/Mx1-Cre mice 2 weeks after the last injection of pI-pC. Thymocytes obtained from age- and sex-matched AML1-IRES-GFP KI mice were used as controls. Gates for GFP-positive cells were determined by comparing their fluorescence with that of WT thymocytes. Numbers indicate the mean ± SE percentage (n = 6).$

MN1-TEL enhances growth of thymocytes and blocks their differentiation. (A) Increased fraction of S-phase cells in MN1-TEL–positive thymocytes. BrdU was injected in MN1-TEL/Mx1-Cre mice 2 weeks after the last injection of pI-pC or PBS, and 4 hours later thymocytes were harvested and analyzed by FCM analysis after staining with an anti-BrdU antibody. Mean ± SE is shown (n = 10). ^*P < .05. (B) Surface marker analysis of GFP-positive thymocytes. Thymocytes were harvested from 6- to 8-week-old MN1-TEL/Mx1-Cre mice 2 weeks after the last injection of pI-pC. Thymocytes obtained from age- and sex-matched AML1-IRES-GFP KI mice were used as controls. Gates for GFP-positive cells were determined by comparing their fluorescence with that of WT thymocytes. Numbers indicate the mean ± SE percentage (n = 6).

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