gp130^flox/flox/TCre bone marrow retains progenitor cells. (A) Quantification of the indicated myeloerythroid colonies that developed from bone marrow or spleen cells suspended in methylcellulose. Data represent the mean ± SD of triplicate determinations and are representative of 3 independent experiments. ^*P < .001 compared with gp130^+/+/TCre. (B) To determine the relative proportions of hematopoietic stem/progenitor cells, Lin^- bone marrow cells were stained with PE–anti-Sca-1 and APC–anti-c-kit and then were analyzed by flow cytometry. The c-kit^HiSca-1⁺, c-kit^HiSca-1^-, and c-kit^LoSca-1^-/Lo populations are gated with open boxes. The percentage of cells in each population is shown. (C) Quantification of the indicated myeloerythroid colonies that developed from 200 sorted Lin^-c-kit^HiSca-1⁺ cells suspended in methylcellulose. Data represent the mean ± SD of triplicate determinations from cells pooled from 5 mice per genotype and are representative of 2 independent experiments.