Triad1 inhibits proliferation of committed myeloid progenitors in liquid cultures. (A) Proliferation of transduced bone marrow cells in liquid medium, indicating dramatic growth inhibition of Triad1-transduced cells compared with empty vector-transduced cells. Cell number at start of culture was set at 100, and relative numbers during culture time (days) were plotted. ⋄ and ▪ represent, respectively, empty vector- and Triad1-transduced cells. (B) Reduced growth rate of Triad1-transduced cells is accompanied by an increase in the percentage of cells in G0/G1 phase (DNA histograms were determined by flow cytometry using propidium iodide staining; Figure S3C). (C) Triad1-transduced cells grown in liquid medium show enhanced annexin V positivity compared with empty vector-transduced cells. (D) Triad1 expression does not result in alterations in the percentage of Mac1-positive cells compared with empty vector-transduced cells. Error bars indicate standard deviations.
