Cancellation of NF-κB activity by nuclear localization of MALT1 in the presence of BCL10. (A) The cells transfected with MALT1-SV40NLS expression vectors were examined by means of immunofluorescence analysis. The protein structure of the MALT1-SV40NLS expression vector is shown below the immunofluorescence image. Numbers below the structures represent the amino acid number of MALT1. (B) COS7 cells (1 × 105) were transfected with NF-κB-dependent luciferase reporter vectors, an internal control vector (phRL SV40), and the following expression vectors: for control, pcDNA3-myc; for MALT1, pcDNA3-MALT1-flag; for MALT1-SV40NLS, pMALT1-flag-SV40NLS; for BCL10, pcDNA3-myc-BCL10; for MALT1 + BCL10, pcDNA3-MALT1-flag and pcDNA3-myc-BCL10; for MALT1-SV40NLS + BCL10, pMALT1-flag-SV40NLS and pcDNA3-myc-BCL10. At 24 hours after transfection, total extracts were used to measure luciferase activity, using the method of the Dual-Luciferase Reporter Assay System (Promega) (top panel) and to detect each of the proteins by means of Western blotting (bottompanel). NF-κB activity was examined 3 times, and one set of representative data is shown. IB indicates immunoblot. Error bars indicate standard deviation.
