Enhanced TNF-α-mediated apoptosis of Fanca^-/- c-kit⁺ cells is blocked by antioxidants and p38 inhibition. (A) Antioxidant and TNF-α-induced apoptosis of c-kit⁺ cells. WT and Fanca^-/- c-kit⁺ cells were pretreated with SeMet or were cultured in normal conditions before 24-hour exposure to 10 ng/mL TNF-α. Cytospins of cells from each experimental group were made before apoptosis was analyzed using a TUNEL assay (n = 3; ^*P ≤ .001). (B) p38 inhibitor and TNF-α-induced apoptosis of c-kit⁺ cells. WT and Fanca^-/- c-kit⁺ cells were pretreated with SB203580 or were cultured in normal conditions before 24-hour treatment with 10 ng/mL TNF-α. Apoptosis was analyzed as described (n = 3; ^*P ≤ .003). Data shown represent the mean of multiple experiments, and error bars represent the SD.