Presence of Rap on the light chain and glycosylation. (A) Western blotting. Lane 1: anti-Rap mAb; lanes 2 to 4: 2L-Rap-hLL1-γ4P in increasing amounts; lanes 5 to 6: hLL1. Two bands corresponding to the fused light chains of 2L-Rap-hLL1-γ4P were clearly detected in lanes 2 to 4 by a mouse anti-Rap mAb, indicating the presence of Rap on both light chains. (B) N-glycosidase treatment of 2L-Rap-hLL1-γ4P. Lane 1: Mr markers; lane 2: untreated sample; lanes 3 to 5: samples treated with excess N-glycosidase loaded in increasing amounts; lane 7.