Effect of DHG on the rate of intrinsic tenase decay. Factor X (200 nM) was added at intervals between 15 seconds (time 0) and 15 minutes after cofactor addition to 0.2 nM factor IXa wild type, 1.0 nM factor VIIIa, 50 μM PC/PS vesicles, 0.15 M NaCl, 20 mM HEPES (pH 7.4), 2 mM CaCl₂, 1 mg/mL BSA, and 0.1% PEG-8000 in the absence (•) and presence of 4 (○) or 6 (▪) nM DHG. The rate of factor X activation (nM/min) by the intrinsic tenase complex was determined as described in “Materials and methods.” Mean values (% control) were plotted with error bars representing ± SD (n = 3). The k_obs for intrinsic tenase decay was 0.12 min^–1, 0.16 min^–1, and 0.19 min^–1 in the presence of 0, 4, or 6 nM DHG, respectively.