Analysis of NPM1 and FLT3-mutant/wt ratio in patient samples. (A) Genescan analysis for NPM1 and FLT3-ITD. Lanes 1 and 2 show examples of an NPM1-wt– and FLT3-ITD–negative case. Lanes 3 through 6 illustrate two patients with mutant NPM1 and FLT3-ITD mutations. Note that in both cases, two independent FLT3-ITD mutations were found. (B) Comparison of NPM1 and FLT3-ITD mutant/wt ratio in 149 samples analyzed for both abnormalities. (C) Comparison of NPM1 and FLT3 in 3 different groups according to the difference between NPM1-mut/wt and FLT3-ITD mut/wt ratio.