![Figure 2. Cell-surface expression of WT and mutated CXCR3 receptors on HEK 293 cells. WT and mutant CXCR3 cell-surface expression on stable transfected HEK 293 cell clones was tested by immunostaining with anti-CXCR3 specific antibodies and analyzed by FACS (black line). Immunostaining with IgG1 isotype control antibodies is in gray line. (A) WT CXCR3. (B) 349stopΔ20 C-tail truncated. (C) 332stopΔ37 C-tail truncated. (D) 332-334L→A C-tail mutations. (E) S245 to A 3i loop mutation. (F) Double-mutant of 332stopΔ37 C-tail truncated and S245 to A 3i loop mutation. (G) HEK 293 cells. Counts indicate relative cell number. A representative histogram of at least 3 experiments performed is presented. (H) Binding of biotinylated I-TAC (mean florescence intensity [MFI]) at different concentrations (nM) to 293 cells expressing the different mutated or truncated CXCR3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/10/10.1182_blood-2004-01-0214/2/zh80100695830002.jpeg?Expires=1735550651&Signature=0uXqmDR5ymTyQoNf-WfIzQQbxwKfAS2SeNj5eb4RSuB65TJXidX9HHi4ZcJ4ftXlFtv0yETHVLg0ZOnJ3UnGwR5XwkpHCLpDZ7YHis6DiNctjEOmKNHswZ7cMYp942xWpuvFvigsr09WN1-WrttnZUarPnl4UdNZaotTjn5y68Y6fx3H~hMRa~MxDVj70WbG~AeFxhoOV3xQr54u5FeWWYKy~Jah95xe2E~vlgFo5zVe-PA7S59iRw3DuSAC9ROlzV8xnk5-UAahFF2HmJNSONFFcDGuyRlPCYZIQGD9r8KcJ0F76~Xa0QXgHecPpK7f-s-r~6kglKS818I3vK8L7A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell-surface expression of WT and mutated CXCR3 receptors on HEK 293 cells. WT and mutant CXCR3 cell-surface expression on stable transfected HEK 293 cell clones was tested by immunostaining with anti-CXCR3 specific antibodies and analyzed by FACS (black line). Immunostaining with IgG1 isotype control antibodies is in gray line. (A) WT CXCR3. (B) 349stopΔ20 C-tail truncated. (C) 332stopΔ37 C-tail truncated. (D) 332-334L→A C-tail mutations. (E) S245 to A 3i loop mutation. (F) Double-mutant of 332stopΔ37 C-tail truncated and S245 to A 3i loop mutation. (G) HEK 293 cells. Counts indicate relative cell number. A representative histogram of at least 3 experiments performed is presented. (H) Binding of biotinylated I-TAC (mean florescence intensity [MFI]) at different concentrations (nM) to 293 cells expressing the different mutated or truncated CXCR3.
