Expression and function of WT CXCR3 and S245A-mutated CXCR3 in Jurkat, YTS, and RBL hematopoietic cells. (A) Cell-surface expression of WT or the S245A mutated CXCR3 in HEK 293, Jurkat, YTS, and RBL cells (black line). Immunostaining with immunoglobulin G₁ (IgG₁) isotype control antibodies is shown in filled histograms. Cell-surface expression of CXCR3 or the S245A-mutated CXCR3 in cells incubated with 1 mg/mL CXCL11 for 1 hour is also shown (gray line). (B-C) The migration of parental-, CXCR3-, and CXCR3-S245A-expressing YTS (B) and Jurkat cells (C) in response to different concentrations of CXCL11 is shown. Each value represents the mean plus or minus SD of 3 independent experiments.