Activation of HTLV-1 Tax reduces CD95-dependent cleavage of Bid. Jurkat ERtax cells were stimulated with 30 μg/mL anti-CD3, 2 μM HT, or both for 24 hours. Surviving cells were isolated by Ficoll gradient centrifugation and stimulated with 200 ng/mL LZ-CD95L for the indicated periods of time. Cell lysates were subjected to Western blot analysis with antibodies against Bid. Expression of tubulin was analyzed as loading control. Induction of c-FLIP was analyzed as positive control for stimulation.