In vivo expression of BDD porcine fVIII from MSCs. (A) Micrograph of BDD porcine fVIII–expressing MSCs bound to dextran microcarrier beads. Image was visualized under an Olympus IX50 inverted microscope equipped with a 4 ×/0.13 objective lens (Olympus, Melville, NY). An Optronics Magnafire camera (Optronics, Goleta, CA) was used to capture images, and Magnafire 2.1 software to acquire and process them. (B) Plasma fVIII activity levels (•) following intraperitoneal transplantation of porcine fVIII–expressing MSCs bound to microcarrier beads into hemophilia A mice (n = 4). Anti–porcine fVIII ELISA (○) and Bethesda titers (▵) are shown (mean ± standard deviation).