Figure 5.
In vivo expression of BDD porcine fVIII under reduced-intensity transplantation conditioning. Plasma fVIII activity levels (symbols) were determined following transplantation of BDD porcine fVIII transgene–modified Sca-1+ cells into hemophilia A mice. Recipient mice were preconditioned with a sublethal dose of TBI (5.5 Gy) and received a transplant of 3 × 105 transduced Sca-1+ cells. Each line represents an individual mouse (n = 5). One mouse died 2 weeks after transplantation due to isoflurane overexposure during blood collection.
