Rottlerin decreases IFN-γ levels in aplastic anemia T cells. We examined the effect of rottlerin on intracellular IFN-γ levels using flow cytometric analysis in PBMCs from aplastic anemia patients (n = 4). In patients with increased IFN-γ levels (3 of 4 patients examined), rottlerin diminished IFN-γ by approximately 50%. A representative experiment of all patients and controls examined is shown. Blue indicates CD4⁺; green, CD8⁺.