Immunoscopy applied to PBMCs and a skin specimen of patient 2: TCR Vβ spectratyping. (A) GeneScan Analysis of PBMCs in patient 2 revealed a monoclonal signature for Vβ2 (shown to be malignant by 2-color flow cytometry analysis), apparent loss of several families (*) and many contracted profiles (**). (B) Gene-Scan Analysis of a skin sample from the same patient revealed a complete loss of all Vβ families, with a single monoclonal peak of a size identical to that of the malignant T-cell clone identified in the blood, indicating the presence of the malignant T-cell clone in the skin and the complete loss of other T cells.