MAPC DLs persist in tissues. Postmortem tissues analysis was performed on 2 to 4 representative animals from each cohort: intravenous delivery with and without NK depletion (B6, Rag2^–/–, Rag2/IL-2Rγc^–/–), intra-arterial delivery (Rag2/IL-2Rγc^–/–), and intravenous or intra-arterial MAPC DLs infused with B10.BR bone marrow into lethally irradiated B6 mice. MAPC DL–derived cells were detected in multiple tissues. Shown here are donor MAPC DL–derived cells in the lung (A), kidney (B), and liver (C) of the B6 mouse, with the highest BLI 30 days after intra-arterial infusion of MAPCs and intravenous infusion of allogeneic bone marrow (original magnification, 200 ×). Donor MAPC DL–derived cells (thin arrows) appeared red as a result of native DsRed2 fluorescence, and nuclei were stained blue with DAPI. Donor MAPC DLs expressed MHC class I in multiple tissues. Shown here are lung cells expressing H2b (green; D) and DsRed2 (red; E) and the superimposition of the green and red pictures (donor MAPC DL cell, thick arrow; recipient cell, thin arrow; original magnification, 400 ×; F).