Steady-state proliferation and survival of CD44^high CD8 T cells are not affected in c-myc^+/– mice. (A) Wt and c-myc⁺/– littermates were injected intraperitoneally with BrdU, and BrdU was added in drinking water for 15 days. BrdU incorporation was measured for CD44^low and CD44^high CD8 T cells in the spleen. Numbers indicate the mean percentages ± SD of BrdU⁺ cells. The results shown are representative of 3 experiments. (B) Intracellular Bcl-2 expression in CD44^low (filled histograms) and CD44^high (empty histograms) CD8 T cells from wt and c-myc⁺/– littermates. Isotype control is shown in dotted lines. Bar graphs show the mean fluorescence intensity (MFI) ± SD of Bcl-2 expression in CD44^low and CD44^high CD8 T cells from wt (white) and c-myc⁺/– (black) mice. The results shown are representative of 3 experiments, and 3 to 5 mice per group were analyzed in each experiment. (C) After 1 hour of incubation at 37°C, the mean percentage ± SD of Annexin V⁺ CD44^low and CD44^high CD8 T cells from wt and c-myc^+/– littermates was determined by flow cytometry.