DC-SIGN ligation in immature MDDCs enhances maturation-dependent IL-10 production. MDDCs were incubated with LPS, TNF-α, Pam3Cys, MR-1, or an isotype-matched antibody (IgG), either alone or in the indicated combinations, for 18 hours in complete medium. After incubation, supernatants from MDDCs were collected and IL-10 content was determined by enzyme-linked immunosorbent assay (ELISA). The data indicate means (SD of triplicate samples from 1 representative experiment of 3 experiments on MDDCs from independent donors).