Two missing regions in the patient's GFTP are required for Golgi localization and transport function. The C-terminal deletion mutants Δ25C and Δ22C lack the last 25 and 22 amino acids, respectively. These constructs bear an N-terminal GFP-tag. The N-terminal deletion mutant Δ37N lacks the first 37 amino acids and bears a C-terminal GFP-tag. Only one of 10 transmembrane domains is shown. Fibroblasts of patient G were transfected with the deletion constructs and analyzed for the GFP-tag and golgin-97 (A) or GFP and AAL (B). Construct Δ37N was compared with a C-terminally tagged wild-type GFTP that was functional and located in the Golgi (not shown). One representative experiment of 3 is shown. Bars represent 10 μm.