Figure 5.
Effect of cationic amino acid deprivation in the medium on differentiation of K562 cells stimulated with sodium butyrate. K562 cells were seeded in 12-well plates at a density of 1 × 105 cells/mL and incubated in culture medium deprived of l-arginine, l-lysine, or l-histidine for 96 hours. Data are shown as the ratio of benzidine-positive cells with respect to that in complete medium stimulated with sodium butyrate as a control. Data represent means of 3 experiments, and error bars indicate SE.