Cluster analysis of differentially expressed genes between DS and non-DS megakaryoblasts; chromosomal localization of genes in cluster analysis. (A) Microarray analysis using the Affymetrix U133A genechip was performed with DS (n = 5) and non-DS (n = 5) AMkL samples and clinically relevant AMkL cell lines (n = 3). Following selection of genes that were present or marginal in either group and filtered for those transcripts with a minimum 2-fold change in expression between DS AMkL and non-DS AMkL, one-way analysis of variance (ANOVA) was used with the Benjamini and Hochberg multiple test correction to control the FDR at 10%, and identified 551 genes as differentially expressed between DS AMkL and non-DS AMkL. Clustering was performed using the median normalized expression data with a Euclidean distance metric and identified 105 genes overexpressed in the DS group and 447 genes overexpressed in the non-DS group. (B) Chromosome localization of the 551 genes analyzed in the cluster analysis.