Figure 1.
Figure 1. Targeted disruption of the mouse Klf6 gene. (A) Genomic structure of the mouse Klf6 gene and targeting vectors. (B) Genotyping of Klf6+/+ and Klf6+/- ES cells by Southern blot analysis using Probe 1 following HindIII digestion of genomic DNA. (C) Genotyping of Klf6+/- and Klf6-/- ES cells by Southern blot analysis using Probe 2 following BamHI digestion. (D) Genotyping of ES cells by PCR. Exon 2 of the Klf6 gene and the neomycin and hygromycin genes were amplified. Klf6-/- ES cells express only the neomycin and hygromycin sequences, but not Klf6 exon 2. In these analyses, genomic DNAs were extracted after depletion of mouse embryonic feeder cells. (E) Expression of the Klf6 gene. After depletion of mouse embryonic feeder cells, total RNAs were extracted and subjected to RT-PCR.

Targeted disruption of the mouse Klf6 gene. (A) Genomic structure of the mouse Klf6 gene and targeting vectors. (B) Genotyping of Klf6+/+ and Klf6+/- ES cells by Southern blot analysis using Probe 1 following HindIII digestion of genomic DNA. (C) Genotyping of Klf6+/- and Klf6-/- ES cells by Southern blot analysis using Probe 2 following BamHI digestion. (D) Genotyping of ES cells by PCR. Exon 2 of the Klf6 gene and the neomycin and hygromycin genes were amplified. Klf6-/- ES cells express only the neomycin and hygromycin sequences, but not Klf6 exon 2. In these analyses, genomic DNAs were extracted after depletion of mouse embryonic feeder cells. (E) Expression of the Klf6 gene. After depletion of mouse embryonic feeder cells, total RNAs were extracted and subjected to RT-PCR.

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